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After 0 h, 24 h, 48 h, 72 h, and 96 h of inoculation, the CCK-8 reaction solution was added according to the instructions of the CCK-8 kit (Beyotime, Shanghai, China), and the absorbance value at the wavelength of 450 nm was detected using an TECAN reader after incubation at 37 C for 1 h

The points below are general boundaries that show up in the literature and in compounding-pharmacy review

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The authors also noted that further optimisation was needed, including work relating to high clearance in mice and off-target activity against monoamine oxidase A