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Equipment and LC-MS/MS conditions Chromatography: The chromatography analysis was performed using a sciex triple quad 6500+ LC-MS/MS equipped with a degasser model DGU-405, controller SCL-40 and CBM-40 LITE, pump LC-40D X3 and LC-40D XS, autosampler SIL-40C X3 and SIL-40C XS, and column oven CTO-40C and CTO-40S for the determination of semaglutide in human plasma samples

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Despite its benign behavior, on clinical grounds the differentiation of LMM may be frequently required from malignant melanoma possibly being characterized by asymmetry, variegated pigmentation from light to dark brown, irregular borders, and large size5, 6