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Rabbit anti-GLP-1R antibody, mouse anti-glial fibrillary acidic protein (GFAP) and mouse anti-alpha smooth muscle actin ( -SMA) were from Abcam PLC (Cambridge, UK), Goat anti-type I collagen antibody from Southern Biotechnology (Birmingham, AL, USA), rabbit antibody against glyceraldehydes-3-phosphate dehydrogenase (GAPDH) from Trevigen (Gaithersburg, MD, USA) and anti-rabbit IgG Alexa488-conjugated antibody, anti-mouse-IgG-Alexa555-conjugated antibody, anti-goat-IgG-Alexa350-conjugated antibody and Hoechst33342 were from Invitrogen (Carlsbad, CA, USA)
When conducting Fourier transform infrared spectroscopy analysis, we took NPs, GLP-1RAs@NPs, and GLP-1RAs@NPs-WYRGRL solutions, centrifuged them to obtain the precipitates, and then freeze-dried the precipitates
Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal fibroblasts
