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pH-sensitive micelles loaded with epirubicin specifically localize to lysosomes and induce dose-dependent alkalization, which impairs autophagic flux and sensitizes cancer cells to the drugs cytotoxic effects [43]
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Pharmacotherapy of obesity: Available medications and drugs under investigation (in Eng)
Protocol [2] Cells at approximately 90% confluence are harvested with 0.1% trypsin/0.01% ethylene diamine tetraacetic acid (EDTA) solution and seeded into a 96-well plate at a density of 210 4 cells/well and grown in RPMI-1640 containing 10% FBS for three days, followed by serum starvation for three days
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