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After excluding drug-derived metabolites, the remaining features were examined for biomarkers of glaucoma with multivariate analysis, including a PCA and an OPLS-DA, with EZinfo software (Waters)

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5.1.1.2 Oxidative stress markers In migraine patients, levels of malondialdehyde (MDA) are significantly elevated, especially in cases of migraine with aura, reflecting increased lipid peroxidation and oxidative stress damage (Table 1)