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Reaction of the exposed thiol with Na 2 S 2 should lead to both persulfide and polysulfide formation (Fig

(ii) Ferroptosis may have occurred in certain anti-inflammatory immune cells in AT, which allows a large increase in pro-inflammatory immune cells, further causing systemic inflammation and insulin resistance, and ultimately leading to the development of obesity

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After washing in PBS, primary antibodies (IgM-PE BioLegend 406507, IgD-APC BioLegend 405713, MARCO Santa Cruz sc-65353/ anti-rat PE Abcam ab97058 and Siglec-1 Biolegend 142417) diluted in PBS 10% FCS were incubated overnight at 4 C