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Post-injury recovery with swelling or redness Recent injuries come with an acute inflammatory phase (redness, heat, swelling)
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For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase
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