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50mg vial with 2.5ml bacteriostatic water: 50mg 2.5ml = 20mg/ml For 1mg dose: 1mg 20mg/ml = 0.05ml = 5 units on U-100 syringe 50mg vial with 2ml bacteriostatic water: 50mg 2ml = 25mg/ml For 1mg dose: 1mg 25mg/ml = 0.04ml = 4 units on U-100 syringe 100mg vial with 5ml bacteriostatic water: 100mg 5ml = 20mg/ml For 2mg dose: 2mg 20mg/ml = 0.1ml = 10 units on U-100 syringe Higher concentrations mean smaller injection volumes but require more precise syringe handling

Immune response induced by oral delivery of Bacillus subtilis spores expressing enolase of Clonorchis sinensis in grass carps (Ctenopharyngodon idellus)