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Real-time quantitative PCR (RT-qPCR) was performed in triplicate on a CFX96 system (Bio-Rad, Hercules, CA, USA) using SYBR Green Mix (Toyobo, Osaka, Japan)
If you experience a life-threatening allergic reaction from a B12 injection, call 911 or go to the nearest emergency room
Early clinical trials indicate artificial pancreas systems are superior to conventional insulin pump strategies, which require input from the user and therefore are prone to human error (Heinemann 2015
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