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It should be noted that treatment of cells with 25HC did not alter the internalization rate of cell surface v3 integrin

Carnitine analogs produced by the gut microbiota can colocalize with carnitine in the white matter of the brain and inhibit carnitine-mediated FAO in cell culture models of mouse CNS white matter, affecting the hosts nervous system, which provides a new perspective for understanding the complexity of the gutbrain axis [252]

It is therefore suggesting that the slight change of relaxation observed in the aorta without endothelium ex vivo might be due to some mechanism unrelated to the direct nitric oxide stimulation which needs further investigation