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To correlate lux-based bioluminescence and bacterial metabolic activity, stationary-phase JE2 and JE2-lux cultures were subcultured at 1:200 in MHB, incubated at 37 C for 3 h with shaking, then treated with 00.5 mM sodium arsenate (Sigma) for 30 min
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If a frozen aliquot is thawed, this is stable for 4 days at 2-80C 9
Because of this, we know the important cold chain operations and regulatory paperwork needs for getting peptides from other countries
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