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To generate plasmid pcDNA3.1/ARCHGEF11 coding for human PDZ RhoGEF for in vitro transcription-translation, PDZ RhoGEF was amplified by PCR by using plasmid mCitrine-YFP-ARHGEF11 as a template and inserted into the pcDNA3.1 zeo (+) vector (Invitrogen) in two steps: first, the nucleotides 11943 were inserted as a KpnI-EcoRI fragment and then the nucleotides 19444566 as an EcoRI-XhoI fragment

The water molecules that make up ice naturally bond at angles of 60 degrees, which is the same angle we find at every vertex of a regular hexagon

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Baicalein resensitizes tamoxifen-resistant breast cancer cells by reducing aerobic glycolysis and reversing mitochondrial dysfunction via inhibition of hypoxia-inducible factor-1alpha