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A Luminex flow cytometer (RRID: SCR_018025) distinguished the antigens by their bead color and quantified the amount of antibody via the level of fluorescence, reported as median fluorescence intensity (MFI)

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LCLT is used as nutritional therapy in the treatment of fatty liver and hemorrhagic shock in pharmaceutical and as a nutritional supplement in agriculture/animal feed to increase the growth rate and reduce the fat rate of aquatic animal