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The photocrosslinking and CuAAC click biotinylation were performed as previously described by our group 52

Its worth noting that much of AOD-9604s proposed functionality stems from early animal and lab studies

Mechanisms whereby SGLT2 inhibition might contribute to cardioprotection include improved glycemic control, blood pressure, and weight, and improved endothelial function and reduced arterial stiffness thereby reducing afterload (62)