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DNA concentration and integrity were assessed with a NanoDrop 2000 (Thermo Fisher Scientific, USA) and agarose gel electrophoresis

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To further investigate the expression of Mtnr1a and Mtnr1b in pulmonary ILC2s we interrogated our bulk RNAseq dataset of purified, melatonin- and vehicle-treated murine ILC2s ( Supplementary Figure 3E )