ECFCs were plated (18 10 3 /well) in EGM-2 medium, supplemented with 2% FBS and incubated at 37 C- 5% CO 2 pictures were acquired at regular intervals at EVOS optical microscope (Thermo Fisher Scientific, Monza, Italy)
Hepatotoxic agents are generally classified into two categories: metabolism-dependent toxins (e.g., paracetamol, methotrexate, ethanol) and direct-acting toxins (e.g., carbon tetrachloride, cadmium)
The counter GSSSG treatment did not increase activation activity of 1 ng/ml HGF ( left orange-bar ), consistent with the earlier observation that GSSSG did not effectively modulate the HGF-c-met interaction (Figure 1e right column, orange bars 4 and 5 ), and hence served as a control trisulfide
The IBS acknowledges integration into the Interdisciplinary Research Institute of Grenoble (IRIG, CEA)