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The excretions of zinc and magnesium were initially similar to baseline and then had a moderate increase by the 9 th dose
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A volume of 5 l of the protein-dye mixture was dispensed into a 384-well microplate (Thermo Scientific, AB1384K), followed by addition of 5 l of C1-27 and 3 l of silicone oil (to avoid evaporation)
In order for the amino acid to be effective it needs to be included as part of a larger weight loss regimen
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