Cells were cultured at 37 C in 5% CO 2 in Dulbeccos modified Eagles medium (DMEM) with high glucose (4.5 g/L) (DM-22, Omega, Tarzana, CA) supplemented with 10% dialysed fetal bovine serum (FBS) (FB-07, Omega, Tarzana, CA), 100 units/mL of penicillin, 100 g/mL of streptomycin (Gemini Bio-Products, Sacramento, CA), and either 0.5 or 2.0 mM L-glutamine (GS-60, Omega, Tarzana, CA)
However, during Ramadan fasting, people find it challenging to consume the medicine and then fast for the next 30-60 minutes
However, the correlation between glutamine and IDD is still unclear
doi: 10.1016/j.neuroimage.2005.02.037 157 HartRPRoseCSHamerRM