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Tissue samples may be tested for changes in specific genes, which can influence the type of treatment that will work best
The growth medium was removed after treatment, and the cells were cultured in 1x MTT (Keygen Biotech, catalog KGA311) in fresh growth medium for 4 h
Treating cells with L-buthionine sulfoximine (BSO), an inhibitor that blocks the synthesis of GSH, renders neuronal SH-SY5Y cells expressing mutant valosin-containing protein (VCP) R487H more susceptible to oxidative stress (Hirano et al., 2015) and increases mutant SOD1 toxicity (Alvarez-Zaldiernas et al., 2016
[30]Kalinhoff C, Caldern NT
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