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After 24 h culture, the transfected cells were seeded into 384-well microtiter plates at a density of 3,000 cells per well in HBSS supplemented with 5 mM HEPES, 0.1% (w/v) BSA or 0.1% (w/v) casein and 0.5 mM 3-isobutyl-1-methylxanthine

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Assemble all required materials: Lyophilized peptide vial Bacteriostatic water for injection (0.9% benzyl alcohol) Sterile syringes (typically 3mL for reconstitution) Alcohol prep pads Sharps disposal container Pro tip: Never use distilled water or sterile water without preservatives for multi-dose peptide vials