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Regular Monitoring Regular check-ups with a healthcare provider can help monitor for side effects and adjust the treatment plan as needed
The amount of vitamin B12 you need depends on your health and how bad the deficiency is
RNA Extraction and Real-time PCR RNA was extracted from 293T using the Qiagen RNeasy mini kit according to the manufacturers instructions
An antibiotic resistance cassette was PCR-amplified from either pFA6 natNT2 or pFA6 kanMX4 plasmids using primers designed to have 4050 base-pairs of homology directly up- and down-stream of the gene to be deleted
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