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Future Research Directions and Unanswered Questions Despite 50+ years since Pickarts initial isolation work, significant mechanistic and clinical questions remain: Receptor identity: GHK-Cus gene regulatory effects are well-documented, but the specific cellular receptors mediating them remain incompletely characterized

T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2

Fat mass and lean mass were determined using DEXA (Hologic Discovery Series W, Waltham, MA, Apex System Software Version 4.6.0.2)

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