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Soluble His-tagged proteins were purified with NiNTA (Qiagen) as previously 56 , and the eluted proteins were dialyzed against buffer H (20 mM TrisHCl, pH 8.0, 100 mM NaCl, 10% glycerol, and 1 mM MgCl 2 )

Figure 6 The association of sarcosine and serine with clinical manifestations in LC In our investigations of metabolite levels associated with symptoms commonly seen in LC patients such as cognitive function, anxiety, chronic pain, and depression in LC patients, we conducted additional analyses

July 7, 2023