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Microbial removal of heavy metals from contaminated environments using metal-resistant indigenous strains
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Immunoblotting and antibodies Whole-cell extracts were prepared by resuspension of cell pellets in lysis buffer (67.5 mM Tris pH 6.8, 25 mM NaCl, 0.5 mM EDTA, 12.5% glycerol, 0.25% SDS and 1 mM DTT)
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