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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C
Vitamin C too offers certain benefits to the skin tones and elasticity, so the combination is especially handy with pigmentation issues (Whelan, 2024)
5FU treatment significantly increases TOM20 staining as shown in Fig
When comparing IV chelation vs oral chelation, its impossible to say if one is objectively better since people have different needs
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