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For example, if 100mg of a drug with a half-life of 60 minutes is taken, the following is estimated: 60 minutes after administration, 50mg remains 120 minutes after administration, 25mg remains 180 minutes after administration, 12.5mg remains 240 minutes after administration, 6.25mg remains 300 minutes after administration, 3.125mg remains

Position 15 (Histidine D-Phenylalanine): Incorporates another D-form amino acid to support stability and reduce enzymatic cleavage

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The plasmid system is relatively stable but has low cutting and editing efficiency, and continuous expression of Cas9 may increase off-target effects