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Briefly, 10 l of serum was incubated with the top 12 abundant serum protein depletion kit (Thermo Scientific Pierce, Cat# 85164) and digested with trypsin on S-Trap column (ProtiFi, NY)

For instance, if there is discoloration, cloudiness, or visible particles in the solution, the vial should be discarded immediately, even if the expiration date has not yet been reached

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