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Research indicates that upon MC1R binding, MT-1 triggers the cAMP signaling cascade, leading to activation of protein kinase A (PKA) and subsequent upregulation of microphthalmia-associated transcription factor (MITF)

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Protein concentration GSSH (g / mg prot)=yVrvVrvCpr =yCpr Sample weight GSSH (g /g)= yVrv(VrvVsvW)= yW Cellamount GSSH (g /106 cell)= yVrv(VrvVsvN)= yN Solution volume GSSH (g / mL)= yVrv/Vs= 2y N: Cell amount,106 Vrv: Total reaction volume, 0.203 mL Vsv: The volume of supernatant was added into the reaction system, 20 L=0.02 mL W: Sample weight, g