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Once the sample is collected, it is sent to a specialized lab

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The sections were added with normal goat serum blocking solution (Sangon) at room temperature for 20 min, and incubated with following diluted primary antibodies, rabbit anti--SMA (5513 51-AP, 1: 300, Proteintech), rabbit anti-VWF (AB7356, 1: 300, Sigma-Aldrich), and rabbit anti-p-p38 MAPK (4511, 1: 300, Cell Signaling Technology) at 4 C overnight

Nevertheless, our multi-layer integrative approach allowed us to decipher some of the fundamental immunometabolic determinants of CAR T cell response in leukemia patients receiving CD19-CAR T cell therapy