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To investigate whether ATP was contained in GLP-1 containing vesicles, we first attempted to perform co-staining for quinacrine and GLP-1, but the permeabilisation and fixation protocols needed for immunofluorescence staining were incompatible with quinacrine staining for ATP

doi: 10.1056/NEJMoa2032183 9 WhartonSFreitasPHjelmesthJKabischMKandlerKLingvayIet al