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Titration analysis of FLIP-G with GSH concentrations from nM to mM resulted in a sigmoid curve and affinity constants ( K d ) was found to be ~282 M (Fig

Unreconstituted peptide: Storage: 28C (refrigerated) or -20C (frozen) Protect from light and moisture Stable for 23 years when properly stored Tolerates brief room-temperature exposure during shipping Reconstituted solution: Storage: 28C (refrigerated) required Protect from light (keep in the original vial or wrap in foil) Stable for 1428 days refrigerated with sterile water Do not freeze the reconstituted solution Discard if the solution becomes cloudy or contains particles Handling precautions: Always use sterile technique Avoid contaminating vials and solutions Use sterile water to extend stability Label vials with the reconstitution date Store away from food and beverages Research Protocol Design When designing protocols, consider these examples