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Our observations are consistent with a previous clinical study in solid tumors where continuous infusion of BSO depleted tumor GSH below 10% of pretreatment levels with minimal systemic toxic effects
The study analyzed fecal and urine samples to assess gut microbiota composition (via 16S rRNA sequencing and FISH) and metabolic profiles (via 1 H-NMR spectroscopy), with behavioral questionnaires and GI symptom diaries
Next, 18 l of the soluble polar metabolite supernatant was transferred to sample vials for analysis by LCMS as previously described 4,28
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