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The protein samples were separated by SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and incubated with primary antibodies for 12 h

Indication: Specifically for vitamin B12 deficiency due to supplemental insufficiency, certain anemias, gastrointestinal malabsorption, and neurological conditions associated with low B12 levels

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The documented benefits span multiple systems - improved sleep quality through pineal restoration, enhanced immune function via thymus support, visible skin improvements from cellular rejuvenation, and 20-40% lifespan extension in animal studies suggesting profound anti-aging potential