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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 g 8.91, 1 g pVSVG, 5 g of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 L of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturers instructions
Alpha-lipoic acid (ALA) is an intracellular redox regulator that reduces OxS, blocks activation of NF-kB and lowers both ADAM17 activity and ACE2 upregulation 529
Il est gnralement considr comme sr de consommer du glutathion dans lalimentation lorsque la production naturelle de lorganisme est insuffisante
against Baseline: F 1.9,7.7 = 20.01, p = 0.001
