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The luminescence of samples and of the GSH standard curve was detected using a Spark Multimode microplate reader (Tecan)
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During development and progression of atherosclerosis, Nrf2 signaling modulates many physiological and pathophysiological processes, like regulation of lipid homeostasis, CD36 gene expression regulation, foam cell formation, macrophage polarization, immunity regulation (Th2 differentiation and inhibition of pro-inflammatory gene expression through NFB down-regulation), redox regulation and inflammation, improvement of endothelial dysfunction, as well as GSH synthesis and utilization (234245)
Factors that accelerate glutathione depletion: Environmental stress: Exposure to pollutants, heavy metals, and UV rays forces the body to use more glutathione for detoxification
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