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To induce chronic liver damage, the mice were treated repeatedly with carbon tetrachloride (CCl 4 , 1 G/kg intraperitoneal) twice per week for 12 months ( 4 /oil injection, blood as well as liver tissue samples were collected, as previously described ( 2.2 Histopathology Hematoxylin and eosin (H&E) as well as Sirius red staining were performed in 5-m-thick paraformaldehyde-fixed paraffin-embedded liver tissue sections using standard protocols
However, since so many phase I enzymes are induced in the cirrhotic liver, the question of which isoenzymes are relevant for the substrates investigated in this study was not addressed in the present study
Further research is necessary to fully characterize its biological mechanisms, safety profile, and potential applications within biomedical science
High-dose vitamin C (1000-3000mg daily) is commonly recommended to complement injectable glutathione protocols
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