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The viability was measured by using Tecan Spark 10 microplate reader at 450 nm according to the manufacturers instructions
This peptide has linearity in the range of 0.1-100 pmol/L and can be detected with high pressure liquid chromatography (HPLC) or ultrasonic spectroscopy
Carter BZ, Gronda M, Wang Z, Welsh K, Pinilla C, Andreeff M , Schober WD, Nefzi A, Pond GR, Mawji IA, Houghten RA, Ostresh J, Brandwein J, Minden MD, Schuh AC, Wells RA, Messner H, Chun K, Reed JC, Schimmer AD
A very recent, 2016 research article compared the topical moisturizing effect of crosslinked HA (not Hylan B gel specifically, but something called Resilient HA or RHA), HMW-HA and LMW-HA and found that "TEWL (that is trans-epidermal water loss, the water that evaporates from the skin) was reduced by 27.8% with RHA, and by 15.6% with HMW HA, but increased by 55.5% with LMW HA." (You can read much more about HMW and LMW HA here in the geeky details section.) All in all, we think Sodium Hyaluronate Crosspolymer is an awesome version of HA , probably superior to traditional versions, so be happy to spot it on the ingredient list
