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M9 minimal medium was selected for all cultivation experiments because it significantly increases the utility and sensitivity of the biosensor used (Kugler et al., 2020)

( The preserved insulinotropic effect of GLP-1 in patients with T2DM may seem incompatible with the almost complete loss of incretin effect in these patients ( In the late 80ties, researchers began looking for the new glucagon-like peptides in the brain, inspired by the reports of immunoreactive glucagon in certain neurons of the brain ( The demonstration of the GLP-1 receptor and its expression on the beta cells was of course consistent with its powerful insulinotropic effects ( Nevertheless, treatment of diabetes was obviously of great interest, but how to do it

The collected scanning profiles were employed for generating the regression equations

Cells were subsequently trypsinized, washed twice with PBS, and resuspended in 0.3 mL of PBS containing 5% fetal bovine serum (FBS) for analysis