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We drew a standard curve using the gradient dilution of the standard product as a template, added the sample, antibody and HRP aggregation reagent, and put it in a 37C water bath, then washed it five times with a washing solution, we added the chromogenic solution and reacted for 10 min, then detected the absorbance of each hole at a wavelength of 450 nm

10.1111/nep.12785 60 IlanN.ElkinM.VlodavskyI

But did you know theres a type of reflux that doesnt cause heartburn (or almost any other symptoms) at all