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Currently, the recommended models for measuring the intestinal permeability of drugs in vitro include in vitro animal intestinal tissue models and monolayer artificially cultured epithelial cell models (Figure 5) [10-12]
Isotope-labelled PGRMC1 proteins for NMR analyses were prepared by growing cells (BL21 (DE3)) in minimal M9 media in H 2 O or 99.9% 2 H 2 O, including ampicillin, metals, vitamins, 15 N-ammonium chloride and 13 C or 12 C glucose as sources of nitrogen and carbon, respectively
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