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Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which inhibits bacterial growth but does not prevent peptide oxidation

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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

Most patients notice initial improvements in sleep quality and recovery within 2 to 4 weeks