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Predicting hepatotoxicity: Reactive metabolite trapping using glutathione and freshly isolated hepatocytes This poster presents our results to date using clozapine (a compound known to be associated with GSH-adduct formation) as substrate and using stable isotope GSH (GSH13C2,15N) to enhance specificity

Between each stage the resin is filtered and washed to remove byproducts, excess reagents and the terminal protecting groups, and the cycle is repeated until the final peptide is cleaved from the resin and purified using chromatography

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Study methods: A 6-month randomised, double-blinded, placebo-controlled trial of oral GSH (250 or 1,000 mg/day) on GSH levels in blood, erythrocytes, plasma, lymphocytes and exfoliated buccal mucosal cells was conducted in 54 non-smoking adults