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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

The ability of both ADSP and AFP to reduce lipid peroxidation, as indicated by decreased MDA levels, is a noteworthy finding, given the critical role of lipid peroxidation in neurodegenerative processes and depression 33

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