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The DNA sequence spanning the CBS gene ARE motif located 4970 bp upstream of the transcript start site and its mutants were cloned into a GFP expressing reporter pEGFP-N1 (PT3054, Clontech) with sequence specific primers (Supplementary Table 1)

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Vitamin B12 is also critical for neurological function

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doi: 10.1016/j.bone.2019.115079 101 TorekovSSHarslfTRejnmarkLEikenPJensenJBHermanAPet al

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Colace is also gentle, but it may be too weak for active constipation

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