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Expi293F cells were grown in Union-293 medium and used for protein expression, with 1 mg of total DNA plasmid and 3 mg of PEI (Biohub, 78PEI40000-1g) per 1 liter of cells at a density of 1.5 2.0 10 6 cells/ml
Full and honest accounts of the process were given in all forums
The current challenge is not inducing further loss, but preventing weight regain
doi: 10.3389/fnmol.2021.797833 Received 19 October 2021 Accepted 18 November 2021 Published 09 December 2021 Volume 14 - 2021 Edited by Chuang-Rung Chang, National Tsing Hua University, Taiwan Reviewed by Shigeru Yanagi, Gakushuin University, Japan
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