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The need for long term use to avoid regaining weight The STEP 4 study revealed two very important findings, the first demonstrated that patients will lose substantial amounts of weight during the 16-week upward titration period but will not achieve their maximum weight loss until taking the full dose for 1 year
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Additionally, while there are no known toxic effects from expired semaglutide, using degraded medications can sometimes lead to increased risks due to contamination or breakdown of chemical components
EC50 values obtained using this method were then used to calculate: sample ratio = sample EC50/EC50 reference peptide-spiked plasma sample and estimated concentration = Known top concentration of peptide- spiked plasma sample/sample ratio
