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At higher temperatures, its vapor pressure increases, meaning more of it will exist as a gas in the headspace of the vial rather than dissolved in the water where it needs to be to inhibit bacterial growth
Refrigerators cycle on and off, causing temperature fluctuations
Standard reconstitution uses 2 mL of bacteriostatic water per 5 mg vial, producing a concentration of 2.5 mg per mL or 0.25 mg per 0.1 mL (10 units on an insulin syringe)
Primers 3 (1), 120
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