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Methods Human lipoaspirates were transplanted subcutaneously into the backs of normal saline-treated (control) or GSH-treated nude mice

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Analytical verification requires reverse-phase HPLC with UV detection at 214-220 nm to confirm retention time consistency and peak purity above 95%, while mass spectrometry (MALDI-TOF or ESI-MS) can confirm the expected molecular ion at 1419.53 Da and identify any truncation or oxidation products

FDA labeling for chronic weight management applies to adults with a BMI of 30 or higher, or a BMI of 27 or higher with at least one weight-related comorbidity such as type 2 diabetes, hypertension, dyslipidemia, or obstructive sleep apnea