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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation

Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy

The molecule entered human clinical trials in the early 2000s