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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

Microarray-style gene expression work referenced in the literature reported shifts across several hundred genes following copper-peptide exposure, spanning categories tied to protein synthesis, cell signaling, and antioxidant defense

The immune system cant make the difference between the bodys own cells and a pathogen, so it responds by creating an inflammatory process

GHK-CU, also known as Copper Peptide, is a naturally occurring tripeptide composed of glycine, histidine, and lysine, bonded with a copper ion